Klingensmith J S, Mehendale H M
J Toxicol Environ Health. 1982 Jul;10(1):121-9. doi: 10.1080/15287398209530236.
The principal objective of this study was to quantitate the anatomical and biochemical parameters associated with the depletion of body fat by the pesticide, chlordecone (CD, or Kepone). Groups of 5 male Sprague-Dawley rats (150-175 g) were fed a control diet or a diet containing 100 ppm CD for 5, 15, or 20 d. After the treatment period, animals were killed by exsanguination. Weight of the period epididymal fat pads was taken as the anatomical marker for the depletion of body fat. Blood levels of acetoacetate, 3-hydroxybutyrate, and nonesterified fatty acids (NEFA) were measured as biochemical as biochemical markers for lipolysis of body fat depots. Serum enzymes (SGPT, ICD) and serum triglycerides were measured to assess liver damage. A consistent and significant difference was observed in the weight of epididymal fat pads between the control and each of the treatment groups. The reduction in epididymal fat reached a maximum of 60% in the CD-fed animals after 20 d. Circulating ketone bodies were not different in any of the treated-animal groups, indicating that CD treatment does not result in metabolic ketosis. Serum triglycerides and NEFA levels were not significantly different in treatment groups versus in controls. Serum transaminases and isocitrate dehydrogenase were not elevated by exposure to CD. These findings indicate that metabolic ketosis is not induced by dietary exposure to CD. Utilization of lipids as energy substrates appears to be the primary underlying mechanism responsible for the loss of body fat observed in CD-treated rats. It appears that CD induces a depletion of body fat stores as a consequence of altered energy balance of the animal.
本研究的主要目的是对与杀虫剂十氯酮（CD，或称开蓬）导致体脂减少相关的解剖学和生化参数进行定量分析。将5组雄性斯普拉格-道利大鼠（体重150 - 175克）分别喂食对照饮食或含100 ppm CD的饮食，持续5、15或20天。在治疗期结束后，通过放血处死动物。附睾周围脂肪垫的重量被用作体脂减少的解剖学标志物。测定血液中乙酰乙酸、3 - 羟基丁酸和非酯化脂肪酸（NEFA）的水平，作为体脂库脂肪分解的生化标志物。测定血清酶（谷丙转氨酶、异柠檬酸脱氢酶）和血清甘油三酯以评估肝脏损伤。在对照组与每个治疗组之间，附睾周围脂肪垫的重量观察到一致且显著的差异。在喂食CD的动物中，20天后附睾脂肪减少最多达60%。在任何处理组动物中，循环酮体均无差异，表明CD处理不会导致代谢性酮症。治疗组与对照组的血清甘油三酯和NEFA水平无显著差异。暴露于CD后血清转氨酶和异柠檬酸脱氢酶未升高。这些发现表明，饮食接触CD不会诱导代谢性酮症。脂质作为能量底物的利用似乎是CD处理大鼠中观察到的体脂减少的主要潜在机制。似乎CD导致动物体脂储备减少是能量平衡改变的结果。
