Chatterjee A K, Ray A D
Jpn J Exp Med. 1982 Feb;52(1):27-32.
The subcellular RNA and protein metabolism have been studied in emetine-treated rats. Emetine treatment for a period of 10 days reduced the incorporation in vivo of [14C]-leucine into proteins of nuclear fraction of liver. This was accompanied by a reduction in the nuclear protein content. The mitochondrial fraction of heart exhibited an increase in the protein content after emetine treatment. This was, however, not accompanied by an increased rate of incorporation into its protein. On the other hand, the nuclear fraction of heart of emetine-treated rats showed a diminution in the rate of incorporation into its protein without having been reduced in protein content. The mitochondrial and microsomal RNA of liver and the microsomal RNA of heart responded to emetine treatment by showing increased levels. The liver RNase activity was reduced after emetine treatment, while the heart RNase remained independent of the treatment. It has been suggested that both synthesis and breakdown of liver proteins are reduced by emetine treatment. The synthesis of liver nuclear proteins was supposedly affected more than was its breakdown by emetine. The synthesis of rapidly turningover proteins of heart nuclear fraction and the catabolism of slowly turningover proteins of its mitochondrial fraction are thought to be reduced by the emetine treatment.
已对吐根碱处理的大鼠的亚细胞RNA和蛋白质代谢进行了研究。用吐根碱处理10天会降低[14C] - 亮氨酸在体内掺入肝脏核部分蛋白质中的量。这伴随着核蛋白含量的降低。吐根碱处理后，心脏的线粒体部分蛋白质含量增加。然而，这并未伴随着其蛋白质掺入率的增加。另一方面，吐根碱处理的大鼠心脏核部分蛋白质掺入率降低，但其蛋白质含量并未降低。肝脏的线粒体和微粒体RNA以及心脏的微粒体RNA在吐根碱处理后水平升高。吐根碱处理后肝脏核糖核酸酶活性降低，而心脏核糖核酸酶不受该处理的影响。有人提出，吐根碱处理会降低肝脏蛋白质的合成和分解。据推测，吐根碱对肝脏核蛋白合成的影响比对其分解的影响更大。吐根碱处理被认为会降低心脏核部分快速周转蛋白质的合成及其线粒体部分缓慢周转蛋白质的分解代谢。
