使用过氧化氢加速Spurr超薄切片的染色。
Use of hydrogen peroxide to accelerate staining of ultrathin Spurr sections.
作者信息
Pfeiffer S W
出版信息
Stain Technol. 1982 May;57(3):137-42. doi: 10.3109/10520298209066605.
PMID:6182645
Abstract
Pretreatment of ultrathin Spurr sections of glutaraldehyde-osmium fixed tissue with hydrogen peroxide significantly reduces the time required to stain with ethanolic uranyl acetate and lead citrate for electron microscopy. Micrographs compare contrast obtained by this method with that obtained using conventional staining times. Reasons for the facilitating action of hydrogen peroxide are suggested.
摘要
用过氧化氢对经戊二醛-锇固定的组织超薄Spurr切片进行预处理，可显著缩短用醋酸铀乙醇溶液和柠檬酸铅进行电子显微镜染色所需的时间。显微照片比较了用这种方法获得的对比度与使用传统染色时间获得的对比度。文中提出了过氧化氢起促进作用的原因。
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