Grattarola M, Carlo P, Viviani R, Nicolini C
Basic Appl Histochem. 1982;26(3):153-61.
Static automated image analysis was applied to study early variations of chromatin structure in Feulgen-stained liver nuclei from rats injected i.p. with a single dose of dimethylnitrosamine (DMNA), a well known hepatocarcinogen. An increase of nuclear area and a correspondent decrease of average optical density (integrated optical density/area) was observed, as compared with controls, in nuclei from rats treated with 5.4 mg/kg of DMNA. These findings, which were comparable with those induced by partial hepatectomy, indicate the existence in DMNA-treated cells of a chromatin DNA relaxation similar to the G0-G1 transition previously described for human diploid fibroblasts stimulated to proliferate. Because similar results were independently obtained by flow microfluorimetry, it seems reasonable to hypothesize that chromatin decondensation could be a prerequisite for cancer induction.
采用静态自动图像分析技术，研究腹腔注射单剂量二甲基亚硝胺（DMNA，一种著名的肝癌致癌物）的大鼠经福尔根染色的肝细胞核中染色质结构的早期变化。与对照组相比，在接受5.4 mg/kg DMNA处理的大鼠细胞核中，观察到核面积增加，平均光密度（积分光密度/面积）相应降低。这些与部分肝切除诱导的结果相当的发现表明，在DMNA处理的细胞中存在染色质DNA松弛现象，类似于先前描述的刺激增殖的人二倍体成纤维细胞的G0-G1期转变。由于通过流式微荧光测定法独立获得了类似结果，因此推测染色质解聚可能是癌症诱导的一个先决条件似乎是合理的。
