Rapaport E, Schroder E W, Kabcenell A K, Black P H
Cancer Res. 1982 Dec;42(12):4918-20.
Short exposure of cultured quiescent cells to micromolar quantities of beta-all-trans-retinoic acid (RA) has been reported to potentiate the effects of phorbol myristate acetate in promoting the transition from the resting to growing states of these cells. Longer periods of exposure to RA result in substantial inhibition of cellular proliferation. We now show that short-term treatment of quiescent Swiss 3T3 cells with RA yields marked increases in uridine phosphorylation and total cellular RNA synthesis as well as 2-deoxyglucose uptake. Upon subsequent treatment of the cells with phorbol myristate acetate, a direct correlation between the comitogenic activity of RA and its stimulation of uridine phosphorylation and RNA synthesis is apparent. The increases in 2-deoxyglucose uptake persist after long-term exposure of the cells to RA when the growth-inhibitory effects of this agent are observed.
据报道，将培养的静止细胞短期暴露于微摩尔量的全反式维甲酸（RA）可增强佛波酯肉豆蔻酸酯促进这些细胞从静止状态向生长状态转变的作用。长时间暴露于RA会导致细胞增殖受到显著抑制。我们现在表明，用RA对静止的瑞士3T3细胞进行短期处理会使尿苷磷酸化、总细胞RNA合成以及2-脱氧葡萄糖摄取显著增加。在用佛波酯肉豆蔻酸酯随后处理细胞后，RA的协同促有丝分裂活性与其对尿苷磷酸化和RNA合成的刺激之间存在明显的相关性。当观察到该试剂的生长抑制作用时，细胞长期暴露于RA后，2-脱氧葡萄糖摄取的增加仍然存在。
