Ley R D
Cancer Res. 1983 Jan;43(1):41-5.
A hyperimmune rabbit antiserum to ultraviolet-irradiated DNA was observed to contain two activities directed against irradiated DNA. Radioimmunoassay studies demonstrated that both antigenic determinants were pyrimidine dimers as evidenced by the reduction in antibody binding to enzymatically photoreactivated irradiated DNA. Enzymatic photoreactivation specifically monomerizes pyrimidine dimers in DNA. Under conditions in which antiserum binding was measured with native DNA, binding was observed with DNA that contained both thymine-thymine and cytosine-containing dimers but not against native DNA that contained only thymine-thymine dimers. Under these assay conditions, competitive binding to irradiated DNA increased as a linear function of fluence of ultraviolet radiation. Under a second set of conditions in which binding was measured with single-stranded DNA, antibodies were bound to DNA which contained only thymine-thymine dimers. The fluence response for the increase in binding to thymine-thymine dimers was nonlinear and increased as a function of fluence squared. Binding to thymine dimers appeared to require bivalent attachment to two dimers that occurred in close proximity.
观察到一种针对紫外线照射DNA的超免疫兔抗血清含有两种针对照射后DNA的活性。放射免疫分析研究表明，两种抗原决定簇均为嘧啶二聚体，这一点可通过酶促光复活照射后DNA的抗体结合减少得到证明。酶促光复活可特异性地使DNA中的嘧啶二聚体单体化。在用天然DNA测量抗血清结合的条件下，观察到与同时含有胸腺嘧啶 - 胸腺嘧啶和含胞嘧啶二聚体的DNA有结合，但与仅含胸腺嘧啶 - 胸腺嘧啶二聚体的天然DNA无结合。在这些检测条件下，与照射后DNA的竞争性结合随紫外线辐射通量呈线性增加。在另一组用单链DNA测量结合的条件下，抗体与仅含胸腺嘧啶 - 胸腺嘧啶二聚体的DNA结合。与胸腺嘧啶 - 胸腺嘧啶二聚体结合增加的通量响应是非线性的，且随通量平方增加。与胸腺嘧啶二聚体的结合似乎需要二价附着于紧密相邻的两个二聚体。
