Itoh T, Kasahara S, Aizu S, Kato K, Takeuchi M, Mori T
Cell Tissue Res. 1982;226(3):469-76. doi: 10.1007/BF00214777.
In the monolayer of an established epithelial cell line from the rat thymus, IT-26R21, characteristic cell aggregates quite similar to Hassall's corpuscles were formed. These aggregates were examined by light and electron microscopy, and immunohistochemically. Their interpretation as Hassall's corpuscles is based n the following observations: (1) The aggregates are formed in the monolayer of cells that greatly resemble medullary epithelial cells of the thymus. (2) They consist of flattened epithelial cells in a concentric pattern with one or ore degenerating cells in the center. (3) Loss of microvilli suggests that these cells are keratinizing. (4) The aggregates show strongly positive reactions in immunofluorescent staining with antikeratin and antiprekeratin. When Hassall's corpuscles increase in size, cellular proliferation is somewhat suppressed. Both in vivo and in vitro, they may be interpreted as an expression of a changing growth pattern in confined spaces and thus seem to have little immunological function.
在源自大鼠胸腺的成熟上皮细胞系IT - 26R21的单层培养物中，形成了与哈氏小体非常相似的特征性细胞聚集体。通过光学显微镜、电子显微镜以及免疫组织化学方法对这些聚集体进行了检查。将它们解释为哈氏小体基于以下观察结果：（1）聚集体在与胸腺髓质上皮细胞极为相似的细胞单层中形成。（2）它们由呈同心模式排列的扁平上皮细胞组成，中心有一个或多个正在退化的细胞。（3）微绒毛的丧失表明这些细胞正在角质化。（4）聚集体在用抗角蛋白和抗前角蛋白进行免疫荧光染色时显示出强阳性反应。当哈氏小体尺寸增大时，细胞增殖会受到一定程度的抑制。无论在体内还是体外，它们都可被解释为在有限空间内生长模式变化的一种表现，因此似乎几乎没有免疫功能。
