Aizu S, Itoh T, Yamamoto T Y
J Microsc. 1981 Nov;124(Pt 2):183-7. doi: 10.1111/j.1365-2818.1981.tb00312.x.
A simple method for whole-cell preparation without using gold or platinum grids as substrata for culture is described. Cells were cultured on formvar film over round pores, each 3 mm in diameter, of Thermonox coverslips. The cells on the formvar coated coverslip are fixed, stained, dehydrated in situ, and introduced into a critical point drying apparatus. A small quantity of 0.2% mesh-cement is applied to slot grids, and they are laid onto the formvar film over the pores of the coverslip. After the grids are removed from the plastic substratum, they are ready for observation under the electron microscope.
本文描述了一种无需使用金或铂网格作为培养底物的全细胞制备简单方法。细胞在Thermonox盖玻片上直径为3毫米的圆形孔上方的福尔马膜上培养。在涂有福尔马的盖玻片上的细胞进行原位固定、染色、脱水，然后放入临界点干燥仪中。在槽式网格上涂抹少量0.2%的网水泥，然后将其放置在盖玻片孔上方的福尔马膜上。从塑料底物上取下网格后，即可在电子显微镜下观察。
