• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用高氯酸作为柱后试剂的高效液相色谱荧光法测定生物源5-羟基吲哚和5-甲氧基吲哚

Fluorimetric determination of biogenic 5-hydroxy- and 5-methoxyindoles by high-performance liquid chromatography using perchloric acid as post-column reagent.

作者信息

Hojo T, Nakamura H, Tamura Z

出版信息

J Chromatogr. 1982 Sep 24;247(1):157-64. doi: 10.1016/s0021-9673(00)84865-7.

DOI:10.1016/s0021-9673(00)84865-7
PMID:6183283
Abstract

A method for the determination of biogenic 5-hydroxy- and 5-methoxyindoles using high-performance liquid chromatography with fluorescence detection was developed. The specific fluorescence of these indoles induced by perchloric acid was utilized as the post-column detection system. The determination limits were 3 pmol for 5-hydroxy-L-tryptophan and 5 pmol for serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (HIAA). The method was applied to the determination of 5-HT and HIAA in rat brain tissues using 5-hydroxygramine as the internal standard.

摘要

建立了一种使用高效液相色谱-荧光检测法测定生物源5-羟基吲哚和5-甲氧基吲哚的方法。利用高氯酸诱导的这些吲哚的特异性荧光作为柱后检测系统。5-羟基-L-色氨酸的测定限为3皮摩尔，血清素（5-HT）和5-羟基吲哚-3-乙酸（HIAA）的测定限为5皮摩尔。该方法以5-羟基禾本科碱为内标，应用于大鼠脑组织中5-HT和HIAA的测定。

相似文献

1
Fluorimetric determination of biogenic 5-hydroxy- and 5-methoxyindoles by high-performance liquid chromatography using perchloric acid as post-column reagent.使用高氯酸作为柱后试剂的高效液相色谱荧光法测定生物源5-羟基吲哚和5-甲氧基吲哚
J Chromatogr. 1982 Sep 24;247(1):157-64. doi: 10.1016/s0021-9673(00)84865-7.
2
High-performance liquid chromatographic determination of indoleamines, dopamine, and norepinephrine in rat brain with fluorometric detection.高效液相色谱法结合荧光检测测定大鼠脑中的吲哚胺、多巴胺和去甲肾上腺素。
Anal Biochem. 1983 Feb 1;128(2):275-80. doi: 10.1016/0003-2697(83)90375-5.
3
A comparison of an improved o-phthalaldehyde fluorometric method and high pressure liquid chromatography in the determination of brain 5-hydroxyindoles of rats treated with L-tryptophan and p-chlorophenyl-alanine.改进的邻苯二甲醛荧光法与高压液相色谱法在测定经L-色氨酸和对氯苯丙氨酸处理的大鼠脑5-羟吲哚中的比较。
Br J Pharmacol. 1981 Jun;73(2):555-61. doi: 10.1111/j.1476-5381.1981.tb10455.x.
4
[Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance iquid chromatography-electrochemical detection].[利用高效液相色谱-电化学检测法测定新生大鼠脑中6-羟基-1-甲基-1,2,3,4-四氢-β-咔啉、5-羟色胺和5-羟吲哚乙酸]
Se Pu. 2009 Mar;27(2):216-9.
5
Determination of six indolic compounds, including melatonin, in rat pineal using high-performance liquid chromatography with serial fluorimetric-electrochemical detection.采用高效液相色谱串联荧光-电化学检测法测定大鼠松果体中包括褪黑素在内的六种吲哚类化合物。
J Chromatogr. 1990 Jun 8;528(1):111-21. doi: 10.1016/s0378-4347(00)82367-1.
6
Determination of 5-hydroxytryptophan, serotonin and 5-hydroxyindoleacetic acid in rat and human brain and biological fluids by reversed-phase high-performance liquid chromatography with electrochemical detection.采用反相高效液相色谱-电化学检测法测定大鼠和人脑以及生物体液中的5-羟色氨酸、血清素和5-羟基吲哚乙酸。
J Chromatogr. 1981 Nov 20;218:663-70. doi: 10.1016/s0021-9673(00)82092-0.
7
Simultaneous micro-determination of serotonin and 5-hydroxyindole-3-acetic acid with 5-hydroxy-N omega-methyltryptamine, as an internal standard, in biological materials by high-performance liquid chromatography with electrochemical detection.采用高效液相色谱-电化学检测法，以5-羟基-Nω-甲基色胺作为内标，同时对生物材料中的血清素和5-羟基吲哚-3-乙酸进行微量测定。
J Chromatogr. 1988 Sep 9;430(2):381-7. doi: 10.1016/s0378-4347(00)83174-6.
8
Simultaneous determination of norepinephrine, serotonin, and 5-hydroxyindole-3-acetic acid in microdialysis samples from rat brain by microbore column liquid chromatography with fluorescence detection following derivatization with benzylamine.采用苄胺衍生化后通过微径柱液相色谱-荧光检测法同时测定大鼠脑微透析样品中的去甲肾上腺素、5-羟色胺和5-羟吲哚-3-乙酸。
Anal Biochem. 2003 Jan 15;312(2):125-33. doi: 10.1016/s0003-2697(02)00435-9.
9
A new mass fragmentographic method for the simultaneous analysis of tryptophan, tryptamine, indole-3-acetic acid, serotonin, and 5-hydroxyindole-3-acetic acid in the same sample of rat brain.一种用于同时分析大鼠脑同一样本中色氨酸、色胺、吲哚-3-乙酸、血清素和5-羟基吲哚-3-乙酸的新型质量碎片分析法。
Anal Biochem. 1979 Jan 1;92(1):233-42. doi: 10.1016/0003-2697(79)90651-1.
10
Serotonin and 5-hydroxyindole-3-acetic acid in human plasma and rat brain determined by liquid chromatography with post-column derivatization and fluorescence detection.采用柱后衍生化液相色谱法和荧光检测法测定人血浆和大鼠脑中的血清素和5-羟基吲哚-3-乙酸。
Clin Chem. 1993 Nov;39(11 Pt 1):2355-6.