Mahmood T, Wall H, Sobus S, Stechschulte D J, Abdou N I
J Allergy Clin Immunol. 1982 Dec;70(6):445-51. doi: 10.1016/0091-6749(82)90007-0.
Leukocyte histamine release and blood mononuclear cell proliferative responses to venoms were evaluated in nine patients with sting anaphylaxis. The effect of venom-activated mononuclear cells on the autologous mononuclear cell proliferative response and venom-induced leukocyte histamine release was also studied before and on maintenance immunotherapy. The mononuclear cell proliferative response was low (specific incorporations less than 2.0) and antigen nonspecific both before and during maintenance immunotherapy. Venom-induced in vitro leukocyte histamine release did not change significantly with immunotherapy. However, when patients were on maintenance immunotherapy, venom-activated mononuclear cells cocultured with autologous leukocytes significantly (p less than 0.05) suppressed venom-induced histamine release. The suppressive effect of mononuclear cells was antigen specific and enhanced by in vitro mononuclear cell activation. Suppression of leukocyte histamine release by mononuclear cells extends the regulatory potential of these cells and may identify an additional mechanism by which immunotherapy protects Hymenoptera-sensitive individuals against systemic anaphylaxis.
在9例蜇伤过敏患者中评估了白细胞组胺释放及血液单核细胞对毒液的增殖反应。在维持免疫治疗前及治疗期间，还研究了毒液激活的单核细胞对自体单核细胞增殖反应和毒液诱导的白细胞组胺释放的影响。在维持免疫治疗前及治疗期间，单核细胞增殖反应均较低（特异性掺入小于2.0）且抗原非特异性。免疫治疗后，毒液诱导的体外白细胞组胺释放无显著变化。然而，当患者进行维持免疫治疗时，与自体白细胞共培养的毒液激活单核细胞显著（p小于0.05）抑制了毒液诱导的组胺释放。单核细胞的抑制作用具有抗原特异性，并通过体外单核细胞激活而增强。单核细胞对白细胞组胺释放的抑制扩展了这些细胞的调节潜能，并可能确定免疫治疗保护膜翅目敏感个体免受全身性过敏反应的另一种机制。
