Mason D Y, Cordell J L, Abdulaziz Z, Naiem M, Bordenave G
J Histochem Cytochem. 1982 Nov;30(11):1114-22. doi: 10.1177/30.11.6183312.
The production of mouse peroxidase:antiperoxidase (PAP) complexes suitable for immunohistological use in conjunction with monoclonal antibodies is described. Three approaches were explored: 1) production of conventional polyclonal PAP complexes; 2) conversion of rabbit PAP to "pseudo-mouse PAP" by incubation with monoclonal mouse anti-rabbit immunoglobulin; 3) formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes prepared by the latter technique gave the best immunohistological labeling reactions, being stable on storage and compatible with a wide range of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of lower molecular weight than conventional PAP complexes (fulfilling theoretical predictions based on the monospecificity of monoclonal antibodies).
抗过氧化物酶（PAP）复合物的制备方法。探索了三种方法：1）制备传统的多克隆PAP复合物；2）通过与单克隆小鼠抗兔免疫球蛋白孵育将兔PAP转化为“假小鼠PAP”；3）由单克隆小鼠抗过氧化物酶形成PAP复合物。通过后一种技术制备的PAP复合物产生了最佳的免疫组织学标记反应，在储存时稳定，并且与多种人单克隆抗体兼容。凝胶过滤显示，单克隆PAP的分子量低于传统PAP复合物（符合基于单克隆抗体单特异性的理论预测）。
