Conrad D H, Wingard J R, Ishizaka T
J Immunol. 1983 Jan;130(1):327-33.
The cross-reactivity of the human IgE receptor with mouse and rat IgE was studied. Using leukocytes from a patient with chronic myelogenous leukemia, in which the mononuclear fraction contained up to 75% basophils, both rat and mouse IgE were found to inhibit the binding of 125I-human IgE to the human basophilic leukemia (HBL cells). About 15-fold more rodent IgE was required for 50% inhibition of binding than unlabeled human IgE (hIgE). Dose-response studies using increasing amounts of rodent vs human 125I-IgE indicated that the HBL cells had about 8000 receptors per cell for hIgE and 5500 receptors per cell for rodent IgE. When the HBL cells were surface labeled with 125I and subsequently solubilized with non-ionic detergent, the labeled hIgE receptor could be isolated by either affinity chromatography on IgE-Sepharose (either human or rodent) or by immunoprecipitation with hIgE and anti-IgE. By SDS-PAGE on 10% gels, the receptor had a m.w. of 58,000 daltons. The solubilized receptors exhibited some rebinding to hIgE-Sepharose, and this rebinding could be inhibited by either human or rodent IgE but not by human IgG. Both the HBL cells and normal human basophils could be passively sensitized with murine IgE anti-DNP for antigen-induced histamine release. The minimum concentration of the mouse IgE antibody for sensitizing normal basophils was 20 to 200 ng/ml. Pretreatment of basophils with human IgE, but not human IgG, abrogated the capacity of the murine IgE antibody to sensitize the cells for histamine release, which indicated that the human and rodent IgE were interacting with the same receptor.
研究了人IgE受体与小鼠和大鼠IgE的交叉反应性。使用来自一名慢性粒细胞白血病患者的白细胞，其中单核细胞部分含有高达75%的嗜碱性粒细胞，发现大鼠和小鼠IgE均能抑制125I-人IgE与人嗜碱性白血病（HBL细胞）的结合。与未标记的人IgE（hIgE）相比，50%抑制结合所需的啮齿动物IgE约多15倍。使用递增剂量的啮齿动物与人类125I-IgE进行的剂量反应研究表明，HBL细胞每细胞有大约8000个hIgE受体和每细胞5500个啮齿动物IgE受体。当HBL细胞用125I进行表面标记，随后用非离子去污剂溶解时，标记的hIgE受体可通过IgE-琼脂糖（人或啮齿动物）亲和层析或用hIgE和抗IgE进行免疫沉淀来分离。通过在10%凝胶上进行SDS-PAGE，该受体的分子量为58,000道尔顿。溶解的受体表现出与hIgE-琼脂糖的一些再结合，并且这种再结合可被人或啮齿动物IgE抑制，但不能被人IgG抑制。HBL细胞和正常人嗜碱性粒细胞均可被鼠抗DNP IgE被动致敏以用于抗原诱导的组胺释放。使正常嗜碱性粒细胞致敏的小鼠IgE抗体的最低浓度为20至200 ng/ml。用人IgE而非人IgG预处理嗜碱性粒细胞可消除鼠IgE抗体使细胞对组胺释放致敏的能力，这表明人和啮齿动物IgE与同一受体相互作用。
