Lyness W H
Life Sci. 1982 Oct 4;31(14):1435-43. doi: 10.1016/0024-3205(82)90004-2.
An assay has been developed for brain tryptophan using reverse-phase liquid chromatography with electrochemical detection. The method simultaneously assays dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), as well as 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA). The method does not require elution from ion exchange resins. After deproteinization and centrifugation samples are injected directly onto the chromatographic column. It was found that small changes in mobile phase pH markedly influenced the retention time of tryptophan while elution of the indoleamines and catecholamines did not change. The assay of these endogenous compounds in a single injection proved not expedient but inexpensive. Values obtained using alumina and ion exchange resins yielded comparable values.
已开发出一种使用反相液相色谱-电化学检测法测定脑内色氨酸的方法。该方法可同时测定多巴胺（DA）及其代谢产物二羟基苯乙酸（DOPAC）和高香草酸（HVA），以及5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）。该方法不需要从离子交换树脂上洗脱。经过脱蛋白和离心后，样品可直接注入色谱柱。结果发现，流动相pH值的微小变化会显著影响色氨酸的保留时间，而吲哚胺和儿茶酚胺的洗脱则没有变化。单次进样测定这些内源性化合物并不方便，但成本较低。使用氧化铝和离子交换树脂获得的值相当。
