Kotake C, Heffner T, Vosmer G, Seiden L
Pharmacol Biochem Behav. 1985 Jan;22(1):85-9. doi: 10.1016/0091-3057(85)90490-3.
A method is described for the separation and quantitation of catecholamines, serotonin, and their major metabolites with use of reverse-phase, ion-pair liquid chromatography with electrochemical detection. This method employs columns packed with a microparticulate C-18 resin, octyl sodium sulfate as the ion-pairing agent, and isocratic elution with a citrate-phosphate buffer containing methanol. Conditions are described for the separation of norepinephrine, normetanephrine, 3-methoxy-4-hydroxyphenylglycol, epinephrine, metanephrine, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, serotonin, and 5-hydroxyindole-3-acetic acid and for their quantitation in extracts of rat brain tissue.
本文描述了一种使用反相离子对液相色谱-电化学检测法分离和定量儿茶酚胺、5-羟色胺及其主要代谢产物的方法。该方法采用填充有微粒C-18树脂的色谱柱，以辛基硫酸钠作为离子对试剂，并使用含甲醇的柠檬酸-磷酸盐缓冲液进行等度洗脱。文中介绍了分离去甲肾上腺素、去甲变肾上腺素、3-甲氧基-4-羟基苯乙二醇、肾上腺素、变肾上腺素、多巴胺、3,4-二羟基苯乙酸、高香草酸、5-羟色胺和5-羟基吲哚-3-乙酸的条件以及在大鼠脑组织提取物中对它们进行定量的方法。
