Dutt M K
Microsc Acta. 1982 Sep;86(3):201-5.
This paper presents methods for specific staining of nuclei with aqueous solutions of celestin blue B and gallocyanine in tissue sections from which RNA has been extracted selectively with concentrated phosphoric acid at 5 degrees C for 20 min or by hydrolysis in 6 N HCl at 28 degrees C for 15 min. It has been found that pH of the freshly prepared celestin blue B dye solution is 3.0 and that of an aqueous solution of gallocyanine is 2.8. These pHs can be lowered to 1.5 with concentrated sulphuric or nitric acid and at this pH staining of the nuclei is possible. But with concentrated sulphuric or nitric acid and at this pH staining of the nuclei is possible. But if the pHs are lowered with concentrated hydrochloric or phosphoric acid, effective use of these dyes is not possible. It has been suggested that some dispersion of the two dyes takes place with concentrated sulphuric or nitric acid which are used to lower the pH. Staining of the nuclei is also possible with an aqueous solution of celestin blue B at pH 3.0 but the same is not possible with gallocyanine at pH 2.8. The absorption spectra of nuclei stained with an aqueous solution of celestin blue B at pH 1.5 and 3.0 are fairly identical, the peak of maximum absorption being at 620 nm. Those of nuclei stained with an aqueous solution of gallocyanine reveal irregular peaks. Possible implications of these findings have been discussed.
本文介绍了用天青石蓝B和镓氰宁水溶液对细胞核进行特异性染色的方法，这些组织切片中的RNA已在5℃下用浓磷酸选择性提取20分钟，或在28℃下于6N盐酸中水解15分钟。已发现新制备的天青石蓝B染料溶液的pH值为3.0，镓氰宁水溶液的pH值为2.8。用浓硫酸或浓硝酸可将这些pH值降至1.5，在此pH值下可对细胞核进行染色。但如果用浓盐酸或浓磷酸降低pH值，则无法有效使用这些染料。有人提出，用于降低pH值的浓硫酸或浓硝酸会使两种染料发生某种分散。在pH值为3.0的天青石蓝B水溶液中也可对细胞核进行染色，但在pH值为2.8的镓氰宁水溶液中则不行。在pH值为1.5和3.0时用天青石蓝B水溶液染色的细胞核的吸收光谱相当一致，最大吸收峰在620nm处。用镓氰宁水溶液染色的细胞核的吸收光谱显示出不规则的峰。已对这些发现的可能影响进行了讨论。
