Dutt M K
Microsc Acta. 1982 May;86(1):59-68.
This communication presents informations on the staining of DNA with basic dyes, such as ethyl violet, Janus green, a new red dye obtained from Janus green, and Giemsa, belonging to different chemical groups. It has been found that DNA of tissue sections from which RNA has been extracted selectively with cold concentrated phosphoric acid can be stained with aqueous solutions of these dyes. Further, DNA of tissue sections from which RNA has been extracted and the sections then hydrolysed in 6 N HCl at 28 degrees C for 15 min can also be stained with aqueous solutions of ethyl violet, Janus green, and Giemsa. Moreover, tissue sections that have been hydrolysed in 6 N HCl and then stained with aqueous solutions of these dyes, including the new red dye obtained from Janus green, reveal well-stained nuclei. The absorption spectra of nuclei stained with aqueous solutions of ethyl violet, Janus green and Giemsa, following the above stated three procedures of staining have been presented. Absorption data of nuclei stained with the new red dye after Feulgen hydrolysis of tissue sections as well as those of nuclei in tissue sections from which RNA has been extracted have also been presented herein. The implications of these findings have been discussed.
本通讯介绍了用碱性染料对DNA进行染色的相关信息，这些碱性染料包括乙基紫、詹纳斯绿、一种由詹纳斯绿制得的新型红色染料以及吉姆萨染料，它们属于不同的化学基团。研究发现，用冷浓磷酸选择性提取RNA后的组织切片中的DNA，可用这些染料的水溶液进行染色。此外，提取RNA后的组织切片，然后在28℃下于6N盐酸中水解15分钟，其DNA也可用乙基紫、詹纳斯绿和吉姆萨染料的水溶液进行染色。而且，在6N盐酸中水解后再用这些染料（包括由詹纳斯绿制得的新型红色染料）的水溶液染色的组织切片，细胞核染色效果良好。文中给出了按照上述三种染色方法用乙基紫、詹纳斯绿和吉姆萨染料的水溶液染色后细胞核的吸收光谱。还给出了组织切片经福尔根水解后用新型红色染料染色的细胞核的吸收数据，以及提取RNA后的组织切片中细胞核的吸收数据。并对这些发现的意义进行了讨论。
