Braak H, Braak E
Neurosci Lett. 1982 Sep 20;32(1):1-4. doi: 10.1016/0304-3940(82)90219-1.
Most of the precipitations that normally fill up Golgi impregnated nerve cells can easily be removed by placing the material in a diluted solution of ammonia for several hours. A very fine scattering of electron-dense particles remains. Preparations processed in this way can be studied electron microscopically, in this way rendering fine structural examination of previously identified neurons possible. The method is applicable for silver chromate Golgi techniques and can be used for all parts of the brain.
通常充满高尔基浸染神经细胞的大部分沉淀物，通过将材料置于稀释的氨溶液中数小时，很容易被去除。留下的是非常细小的电子致密颗粒散射。以这种方式处理的标本可以进行电子显微镜研究，从而能够对先前鉴定的神经元进行精细结构检查。该方法适用于铬酸银高尔基技术，可用于大脑的所有部位。
