A simple procedure for electron microscopy of Golgi-impregnated nerve cells.

Most of the precipitations that normally fill up Golgi impregnated nerve cells can easily be removed by placing the material in a diluted solution of ammonia for several hours. A very fine scattering of electron-dense particles remains. Preparations processed in this way can be studied electron microscopically, in this way rendering fine structural examination of previously identified neurons possible. The method is applicable for silver chromate Golgi techniques and can be used for all parts of the brain.