Stolc V
Biochim Biophys Acta. 1982 Nov 17;721(3):236-9. doi: 10.1016/0167-4889(82)90074-x.
The content of adenosine 3',5'-monophosphate in human mononuclear leukocytes was enhanced 3-5-times by venoms obtained from African toad (Bufo africanus), American toad (Bufo americanus), Colorado river toad (Bufo arenarum) and Marine toad (Bufo marinus) at 25 micrograms/ml for 5 min of incubation at 37 degrees C. The maximum stimulation was observed after 1-5 min of incubation. The half-maximal stimulation was observed at 0.1 micrograms/ml venom obtained from Colorado river toad (Bufo arenarum). The increased content of adenosine 3',5'-monophosphate in the mononuclear leukocytes persisted without significant change for at least 30 min of incubation at 37 degrees C.
在37℃下孵育5分钟，浓度为25微克/毫升的非洲蟾蜍（Bufo africanus）、美洲蟾蜍（Bufo americanus）、科罗拉多河蟾蜍（Bufo arenarum）和海蟾蜍（Bufo marinus）的毒液可使人类单核白细胞中3',5'-单磷酸腺苷的含量提高3至5倍。孵育1至5分钟后观察到最大刺激作用。从科罗拉多河蟾蜍（Bufo arenarum）获得的毒液浓度为0.1微克/毫升时观察到半数最大刺激作用。单核白细胞中3',5'-单磷酸腺苷含量的增加在37℃下孵育至少30分钟后持续存在且无显著变化。
