Young C R, Atassi M Z
J Immunogenet. 1982 Oct;9(5):343-51. doi: 10.1111/j.1744-313x.1982.tb00992.x.
Previously, it was reported that the immune response to myoglobin (Mg) was under genetic control, with the response to each site being under separate Ir-gene control. Here we have investigated the effect of antigen dose on the control of the antibody response to the five antigenic sites of sperm-whale Mb to determine whether or not the overcoming of genetic control by antigen dose has a uniform effect on all five antigenic sites. The antibody response to sperm whale myoglobin (Mb) and its five antigenic was measured in the following inbred strains of mice, C57BL/6J, AKR and SWR/J. These strains of mice are low responders to Mb following immunization with 50 micrograms, responding only to site 4. After immunization with 200 micrograms Mb: C57BL/6J mice are high responders to Mb and respond to antigenic sites 1, 3, 4 and 5; AKR mice are high responders to Mb and respond to antigenic sites 1 and 4; SWR/J mice are high responders to Mb and respond to all five antigenic sites. It was concluded that the genetic control of the immune response to Mb and its synthetic antigenic sites is dependent on antigen dose. Also, these studies have enabled us for the first time to separate the response to site 1 from the response to site 2 and thus have conclusively established that sites 1 and 2 are controlled by separate Ir-genes.
此前有报道称，对肌红蛋白（Mg）的免疫反应受基因控制，对每个位点的反应受独立的Ir基因控制。在此，我们研究了抗原剂量对抹香鲸肌红蛋白五个抗原位点抗体反应控制的影响，以确定抗原剂量克服基因控制对所有五个抗原位点是否具有一致的作用。在以下近交系小鼠C57BL/6J、AKR和SWR/J中测量了对抹香鲸肌红蛋白（Mb）及其五种抗原的抗体反应。这些品系的小鼠在用50微克Mb免疫后对Mb反应较弱，仅对位点4有反应。在用200微克Mb免疫后：C57BL/6J小鼠对Mb是高反应者，对抗原位点1、3、4和5有反应；AKR小鼠对Mb是高反应者，对抗原位点1和4有反应；SWR/J小鼠对Mb是高反应者，对所有五个抗原位点都有反应。得出的结论是，对Mb及其合成抗原位点的免疫反应的基因控制取决于抗原剂量。此外，这些研究首次使我们能够将对位点1的反应与对位点2的反应区分开来，从而最终确定位点1和2由独立的Ir基因控制。
