Sobhon P, Tanphaichitr N, Chutatape C, Vongpayabal P, Panuwatsuk W
J Exp Zool. 1982 Nov 1;223(3):277-90. doi: 10.1002/jez.1402230309.
Human sperm chromatin was decondensed by treating the purified sperm heads with Sarkosyl for 60 minutes followed with dithiothreitol (DTT) for 20 minutes and overnight. Following Sarkosyl treatment all histones and nonhistones were removed; the remaining nucleoprotamines in the sperm heads exhibited two levels of higher-order structure in the forms of 900-1200 A thick and 380-520 A thin knobby cords, which were randomly coiled. Subsequent treatment with DTT resulted in the dissociation of the 380-520 A cords into subunits of 180-210 A fibers, which were further decondensed into beads-on-a-string structure with diameter of the beads about 120-150 A.
通过用十二烷基肌氨酸钠处理纯化的精子头部60分钟，随后用二硫苏糖醇（DTT）处理20分钟并过夜，使人精子染色质解聚。十二烷基肌氨酸钠处理后，所有组蛋白和非组蛋白都被去除；精子头部剩余的核精蛋白呈现出两种高级结构水平，形式为900 - 1200埃厚和380 - 520埃细的有节的索，它们随机盘绕。随后用DTT处理导致380 - 520埃的索解离成180 - 210埃纤维的亚基，这些亚基进一步解聚成串珠状结构，珠子直径约为120 - 150埃。
