Rosa F, Fellous M
C R Seances Acad Sci III. 1982 Oct 11;295(5):359-64.
Human alpha and beta interferons increase the rate of class I human MHC antigens mRNA HLA-A, B, C and beta 2-microglobulin (beta 2m) in most human cells studied so far. The present work studies the effect of those interferons upon cell lines which do not express class I antigen on their membrane: K 562 et Daudi. If beta 2m mRNA is enhanced in K 562 cell by both alpha and beta interferons, those interferons are not able to promote the de novo synthesis of HLA-A, B, c mRNA, which is not detectable in this cell line. On the other hand, HLA-A, B, C mRNA, which is present in Daudi cell, is amplified by both alpha and beta interferons. Furthermore, we could show that, if it has not been possible to detect mature beta 2m protein either in the cytoplasm or on Daudi membrane, there is a poly A+ RNA in it, which hybridizes with a cDNA probe specific for beta 2m.
到目前为止，在大多数被研究的人类细胞中，人α干扰素和β干扰素可提高人类Ⅰ类主要组织相容性复合体（MHC）抗原mRNA（HLA-A、B、C）以及β2-微球蛋白（β2m）的水平。本研究探讨了这些干扰素对细胞膜上不表达Ⅰ类抗原的细胞系（K562和Daudi细胞系）的影响。α干扰素和β干扰素均可提高K562细胞中β2m mRNA的水平，但这两种干扰素均无法促进该细胞系中HLA-A、B、C mRNA的从头合成，在此细胞系中无法检测到HLA-A、B、C mRNA。另一方面，Daudi细胞中存在的HLA-A、B、C mRNA可被α干扰素和β干扰素扩增。此外，我们还发现，尽管在Daudi细胞的细胞质或细胞膜上均未检测到成熟的β2m蛋白，但细胞中存在一种多聚腺苷酸（poly A+）RNA，它可与β2m特异性cDNA探针杂交。
