干扰素对HLA - DR信使核糖核酸和细胞表面抗原的差异调节 - Suppr

Rosa F, Hatat D, Abadie A, Wallach D, Revel M, Fellous M

Immunogénétique Humaine, Institut Pasteur, Université Paris VII, France.

EMBO J. 1983;2(9):1585-9. doi: 10.1002/j.1460-2075.1983.tb01628.x.

Human interferons-alpha, -beta and -gamma enhance HLA-DR mRNAs in all the human lymphoblastoid and melanoma cell lines studied. The increase concerns both alpha and beta chain mRNAs. Moreover, we show that immune interferon-gamma preferentially enhances class II MHC mRNA. This effect of IFN-gamma on the synthesis of alpha and beta HLA-DR chains has been also analysed by immunoprecipitation. It is abolished by a monoclonal antibody directed against human IFN-gamma. The effect of interferon on the cell surface level of HLA-DR molecules does not always correspond to the enhancement of HLA-DR mRNA. Our experiments suggest that this discrepancy between the enhancement of HLA-DR mRNA and cell surface antigen might be due to a constitutively high level of the corresponding antigens on several of the human cells studied.

在所有研究的人类淋巴母细胞系和黑色素瘤细胞系中，人α-干扰素、β-干扰素和γ-干扰素均能增强HLA-DR mRNA的表达。这种增加涉及α链和β链mRNA。此外，我们发现免疫干扰素-γ优先增强II类MHC mRNA的表达。通过免疫沉淀法也分析了IFN-γ对α链和β链HLA-DR合成的影响。该作用可被抗人IFN-γ单克隆抗体消除。干扰素对HLA-DR分子细胞表面水平的影响并不总是与HLA-DR mRNA的增强相对应。我们的实验表明，HLA-DR mRNA增强与细胞表面抗原之间的这种差异可能是由于在所研究的几种人类细胞中相应抗原的组成性高水平所致。