Miles R M, Parker J L, Jones R T, Dahlberg M J, Yunginger J W
J Allergy Clin Immunol. 1983 Jan;71(1 Pt 1):36-9. doi: 10.1016/0091-6749(83)90544-4.
We have previously isolated a purified Alternaria fraction (Alt-I) by means of immunochemical (RAST) assays to document allergenicity. In the present study we investigated the allergenicity of Alt-I by in vivo and in vitro biologic tests in a selected group of 16 nonimmunized Alternaria-sensitive patients, all of whom had positive skin tests to crude Alternaria extract; a control group of eight nonallergic individuals was also studied. Intradermal skin-test titration endpoints to Alt-I ranged from 60 pg/ml to 60 ng/ml in the Alternaria-sensitive patients. Alt-I induced greater than 30% in vitro leukocyte histamine in all Alternaria-sensitive patients at submicrogram concentrations. Alt-I did not produce positive skin tests or induce significant leukocyte histamine release in nonallergic individuals. In 14 of 16 Alternaria-sensitive patients bronchoprovocation testing produced greater than 20% fall in forced expiratory volume in one second at Alt-I test concentrations of 0.6 to 60 micrograms/ml. In a separate prospective study of 100 unselected individuals, concordant skin-test reactions were noted to crude Alternaria extract and Alt-I in 96 instances. These results confirm that the Alt-I fraction is a biologically active, major allergenic component of crude Alternaria extract.
我们之前通过免疫化学（放射变应原吸附试验）检测分离出一种纯化的链格孢菌组分（Alt-I），以证明其致敏性。在本研究中，我们对一组选定的16名未免疫的链格孢菌敏感患者进行了体内和体外生物学试验，研究Alt-I的致敏性，所有这些患者对链格孢菌粗提物的皮肤试验均呈阳性；还研究了一个由8名非过敏个体组成的对照组。在链格孢菌敏感患者中，Alt-I的皮内皮肤试验滴定终点范围为60 pg/ml至60 ng/ml。在亚微克浓度下，Alt-I在所有链格孢菌敏感患者中诱导的体外白细胞组胺释放均超过30%。Alt-I在非过敏个体中未产生阳性皮肤试验结果或诱导显著的白细胞组胺释放。在16名链格孢菌敏感患者中的14名中，当Alt-I测试浓度为0.6至60微克/毫升时，支气管激发试验导致一秒用力呼气量下降超过20%。在另一项对100名未经过筛选的个体进行的前瞻性研究中，在96例中观察到对链格孢菌粗提物和Alt-I的皮肤试验反应一致。这些结果证实，Alt-I组分是链格孢菌粗提物的一种具有生物活性的主要致敏成分。
