Chen W W
J Med Genet. 1982 Dec;19(6):433-6. doi: 10.1136/jmg.19.6.433.
Cultivated cells obtained by amniocentesis for antenatal diagnosis were examined for the presence of keratin filaments by immunofluorescent staining techniques. In primary cultures, cells in fibroblast type colonies do not possess keratin filaments whereas cells in epithelial type colonies show positive staining of keratin fibres. The majority of cells in amniotic fluid type colonies also stain positively with antikeratin antibody. After the primary cells have been subcultured, most of them appear fibroblastic yet stain positively with antikeratin antibody. The patterns formed by these stained fibres resemble those seen in primary cultures of amniotic fluid type cells. These results indicate that the amniotic fluid type cells (which predominate in primary cultures) and the majority of cells in subcultures (routinely used for antenatal diagnosis of genetic metabolic disorders) are epithelial in origin.
通过羊膜穿刺术获取用于产前诊断的培养细胞，采用免疫荧光染色技术检测角蛋白丝的存在。在原代培养中，成纤维细胞型集落中的细胞不具有角蛋白丝，而上皮细胞型集落中的细胞对角蛋白纤维呈阳性染色。羊水型集落中的大多数细胞也用抗角蛋白抗体呈阳性染色。原代细胞传代培养后，大多数细胞呈成纤维细胞样，但用抗角蛋白抗体呈阳性染色。这些染色纤维形成的模式类似于羊水型细胞原代培养中所见的模式。这些结果表明，羊水型细胞（在原代培养中占主导）和传代培养中的大多数细胞（常规用于遗传代谢疾病的产前诊断）起源于上皮细胞。
