Simpson N E, Bone M, Girard K
Prenat Diagn. 1982 Oct;2(4):273-80. doi: 10.1002/pd.1970020406.
A quantitative method for cholinesterases in amniotic fluid using the non-specific substrate alpha naphthyl acetate and the cholinesterase-specific inhibitor, eserine, is described. This assay was used to test 671 samples of amniotic fluid. The diagnoses for fetal ONTDs, based on the levels of AChE + ChE, were compared with those made for the same samples by the AFP method. Correct diagnoses were made by both methods with amniotic fluid from 35 women carrying fetuses with ONTDs and 631 carrying normal fetuses. There were five false-positive test results for normal fetuses by both methods when the cut-off points were 5 standard deviations above the mean for AFP and above the upper limit of the normal range (7.5 milliunits) for cholinesterase (AChE + ChE). None of the false-positive samples from either method had the acetylcholinesterase band of activity characteristic of ONTDs after gel electrophoresis. In addition to the above 671 samples, 37 pregnancies with serious fetal abnormalities other than ONTDs were tested. Two were identified by both the AFP and AChE + ChE methods, two more by AFP assay and one other by the AChE + ChE assay.
描述了一种使用非特异性底物α-萘乙酸和胆碱酯酶特异性抑制剂毒扁豆碱测定羊水胆碱酯酶的定量方法。该检测方法用于检测671份羊水样本。将基于乙酰胆碱酯酶（AChE）+胆碱酯酶（ChE）水平对胎儿开放性神经管缺陷（ONTDs）的诊断结果与通过甲胎蛋白（AFP）方法对相同样本所做的诊断结果进行比较。对于35名怀有患ONTDs胎儿的孕妇的羊水以及631名怀有正常胎儿的孕妇的羊水，两种方法均做出了正确诊断。当临界值设定为高于AFP平均值5个标准差以及高于胆碱酯酶（AChE + ChE）正常范围上限（7.5毫单位）时，两种方法对正常胎儿均有5例假阳性检测结果。凝胶电泳后，两种方法的假阳性样本均未出现ONTDs特有的乙酰胆碱酯酶活性条带。除上述671份样本外，还对37例患有除ONTDs之外严重胎儿异常的妊娠进行了检测。两种胎儿异常情况通过AFP和AChE + ChE方法均得以识别，另外两种通过AFP检测识别，还有一种通过AChE + ChE检测识别。
