Jacobsen H, Epstein D A, Friedmann R M, Safer B, Torrence P F
Proc Natl Acad Sci U S A. 1983 Jan;80(1):41-5. doi: 10.1073/pnas.80.1.41.
The double-stranded RNAs (I)n X (C)n and (A)n X (dUfl)n (dUfl is 2'-fluoro-2'-deoxyuridylic acid) have been compared as inhibitors of translation in cell-free systems from interferon-treated mouse L cells and from rabbit reticulocytes. In the interferon-treated mouse L-cell system, both double-stranded RNAs stimulated kinase activity, leading to phosphorylation of protein P1 and eukaryotic initiation factor 2 alpha (eIF-2 alpha), but only (1)n X (C)n activated the (2'-5')-oligoadenylate synthetase. Moreover, in this system, (I)n X (C)n, but not (A)n X (dUfl)n, inhibited translation. Both (A)n X (dUfl)n and (I)n X (C)n also activated the rabbit reticulocyte kinase to phosphorylate protein P1 and eIF-2 alpha, but, in contrast to mouse L-cell systems, both (A)n X (dUfl)n and (I)n X (C)n were potent inhibitors of translation in reticulocyte lysates. These results indicate that protein P1 and eIF-2 alpha phosphorylation are not sufficient to cause inhibition of protein synthesis in interferon-treated mouse L-cell extracts. They further suggest that protein synthesis inhibition by (I)n X (C)n in extracts of interferon-treated L cells correlates better with activation of (2'-5')-oligoadenylate synthetase than with activation of the protein P1 and eIF-2 alpha kinase.
已对双链RNA（I）nX（C）n和（A）nX（dUfl）n（dUfl为2'-氟-2'-脱氧尿苷酸）作为来自经干扰素处理的小鼠L细胞和兔网织红细胞的无细胞系统中翻译抑制剂进行了比较。在经干扰素处理的小鼠L细胞系统中，两种双链RNA均刺激激酶活性，导致蛋白质P1和真核起始因子2α（eIF-2α）磷酸化，但只有（I）nX（C）n激活（2'-5'）-寡腺苷酸合成酶。此外，在该系统中，（I）nX（C）n而非（A）nX（dUfl）n抑制翻译。（A）nX（dUfl）n和（I）nX（C）n均还激活兔网织红细胞激酶使蛋白质P1和eIF-2α磷酸化，但与小鼠L细胞系统相反，（A）nX（dUfl）n和（I）nX（C）n在网织红细胞裂解物中均是有效的翻译抑制剂。这些结果表明，蛋白质P1和eIF-2α磷酸化不足以导致经干扰素处理的小鼠L细胞提取物中蛋白质合成的抑制。它们进一步表明，经干扰素处理的L细胞提取物中（I）nX（C）n对蛋白质合成的抑制与（2'-5'）-寡腺苷酸合成酶的激活比与蛋白质P1和eIF-2α激酶的激活相关性更好。
