Mourot J, Corring T, Charles M
Reprod Nutr Dev (1980). 1980;20(4B):1267-71.
This study reports the effect of long-term (8 days) addition of bile salts in the duodenal lumen of the pig in which the bile enterohepatic cycle was fully maintained. Seventeen pigs were fitted with permanent fistulae in the bile duct and the duodenum. The bile collected in 9 pigs was returned to the intestine, while that of the other 8 pigs was returned after a 30 p. 100 excess in bile salts, supplied by lyophilized porcine bile, had been added. At the end of the 8-day experimental period, the enzymes were determined in the fresh total pancreatic glands of all the pigs. The results showed no significant differences in lipase, colipase, amylase, chymotrypsin or trypsin activities in either group of animals. The existence of an in vivo relationship between bile salts and colipase secretions is hypothesized.
本研究报告了在猪的十二指肠腔内长期（8天）添加胆盐的效果，在此过程中胆汁肝肠循环得以充分维持。17头猪在胆管和十二指肠处安装了永久性瘘管。9头猪收集的胆汁被回输到肠道，而另外8头猪收集的胆汁在添加了由冻干猪胆汁提供的过量30%的胆盐后被回输。在为期8天的实验期结束时，测定了所有猪新鲜胰腺全腺中的酶。结果显示，两组动物的脂肪酶、辅脂肪酶、淀粉酶、糜蛋白酶或胰蛋白酶活性均无显著差异。推测胆盐与辅脂肪酶分泌之间存在体内关系。
