异丁基甲基黄嘌呤对章鱼视网膜光反应及磷脂酰肌醇磷酸化的影响。

The effect of isobutylmethylxanthine on the photoresponse and phosphorylation of phosphatidylinositol in octopus retina.

作者信息

Yoshioka T, Inoue H, Takagi M, Hayashi F, Amakawa T

出版信息

Biochim Biophys Acta. 1983 Jan 4;755(1):50-5. doi: 10.1016/0304-4165(83)90271-4.

Abstract

The effect of isobutylmethylxanthine on the photoresponse and the phosphorylation of phosphatidylinositol was studied. The recovery of photoresponse after a bright flash was greatly altered by the addition of isobutylmethylxanthine. Phosphatidylinositol phosphorylation, assessed by the incorporation of label from [gamma-32P]ATP, was influenced by isobutylmethylxanthine. Incorporation of 32P was decreased into diphosphoinositide and triphosphoinositide, and increased into phosphatidic acid. These changes were interpreted by introducing a parameter, phi, calcium charge of the plasma membrane.

摘要

研究了异丁基甲基黄嘌呤对光反应和磷脂酰肌醇磷酸化的影响。添加异丁基甲基黄嘌呤后，强光闪光后光反应的恢复发生了很大变化。通过[γ-32P]ATP的放射性标记掺入评估的磷脂酰肌醇磷酸化受到异丁基甲基黄嘌呤的影响。32P掺入二磷酸肌醇和三磷酸肌醇减少，而掺入磷脂酸增加。通过引入一个参数，即质膜钙电荷（phi）来解释这些变化。