Eishi Y, Takemura T, Suzuki K, Hatakeyama S
Bull Tokyo Med Dent Univ. 1982 Jun;29(2):47-52.
Staining solutions of acid phosphatase and esterase group (naphthol-AS-D-chloroacetate esterase, naphthol-AS-acetate esterase and alpha-naphthyl-acetate esterase) were stored for 6 months using the freezing facilities, and the staining results were compared with those of each set of controls. The results showed that the staining solutions of all enzymes were stable for long-term preservation (at least 6 months) in the deep freezer (-80 degrees C) when they were stored appropriately in separated forms. In such a freezing procedure, the staining results for each enzyme did not show any decrease of positive intensity nor any non-specific staining.
酸性磷酸酶和酯酶组（萘酚 - AS - D - 氯乙酸酯酶、萘酚 - AS - 乙酸酯酶和α - 萘乙酸酯酶）的染色液使用冷冻设备保存6个月，并将染色结果与每组对照进行比较。结果表明，当所有酶的染色液以分开的形式妥善保存时，在深冻冰箱（-80摄氏度）中可长期稳定保存（至少6个月）。在这种冷冻过程中，每种酶的染色结果均未显示阳性强度降低或出现非特异性染色。
