使用单克隆抗体检测和亲和纯化β-内啡肽前体

Detection and affinity purification of beta-endorphin precursors using a monoclonal antibody.

作者信息

Thorpe R, Spitz L, Spitz M, Austen B M

出版信息

FEBS Lett. 1983 Jan 10;151(1):105-10. doi: 10.1016/0014-5793(83)80353-6.

Abstract

A monoclonal antibody to porcine beta-lipotropin has been produced which binds to the N-terminal (gamma-lipotropin) portion of the molecule. The antibody can be used to detect beta-lipotropin as well as other beta-endorphin precursors (predominantly a Mr 38 000 polypeptide) using radiobinding assay or the immunoblotting technique. Purification of the peptides can be readily achieved by affinity chromatography using the monoclonal antibody covalently bound to Sepharose 4B. As the antibody recognises the N-terminal part of beta-lipotropin, it can be used to detect and purify beta-lipotropin and other beta-endorphin precursors in the presence of beta-endorphin.

摘要

已制备出一种针对猪β-促脂素的单克隆抗体，它能与该分子的N端（γ-促脂素）部分结合。使用放射结合测定法或免疫印迹技术，该抗体可用于检测β-促脂素以及其他β-内啡肽前体（主要是一种分子量为38000的多肽）。通过使用共价结合到琼脂糖4B上的单克隆抗体进行亲和层析，可轻松实现肽的纯化。由于该抗体识别β-促脂素的N端部分，因此在存在β-内啡肽的情况下，它可用于检测和纯化β-促脂素及其他β-内啡肽前体。