Fein A, Toder V, Nebel L
Dev Comp Immunol. 1982 Fall;6(4):747-54.
Rat blastocysts after 3, 24 or 72 hours in culture, were exposed to antiserum against rat embryo and mouse placenta in the presence of complement. The concomitant exposure damaged the trophoblastic cells and the inner cell mass. Differences were not observed in the nature and strength of the damage between blastocysts with or without zona pellucida. When the cultured blastocysts were exposed first to antisera and only later to complement, the damage seemed to be selective. In the 3 and 24 hour cultured blastocysts, only the trophoblastic cells were destroyed but not the inner cell mass. In the 72 hour cultures, the damage was inflicted to all cells including the inner cell mass, exposed after spreading of trophoblastic cells. The anti-rat embryo serum and the anti-mouse placenta serum had similar effects in low dilutions. In high dilution, the anti-rat embryo serum was more potent. Isolated inner cell masses were cultured up to 72 hours but did not attach to the dish nor spread. Nevertheless, the cells survived, proliferated and differentiated into an outer layer of seemingly endodermal cells.
将培养3、24或72小时后的大鼠囊胚，在补体存在的情况下，暴露于抗大鼠胚胎和抗小鼠胎盘的抗血清中。同时暴露会损伤滋养层细胞和内细胞团。有或没有透明带的囊胚在损伤的性质和强度上未观察到差异。当培养的囊胚先暴露于抗血清，随后才暴露于补体时，损伤似乎具有选择性。在培养3小时和24小时的囊胚中，仅滋养层细胞被破坏，而内细胞团未被破坏。在培养72小时的囊胚中，在滋养层细胞铺展后暴露的所有细胞，包括内细胞团，均受到损伤。低稀释度时，抗大鼠胚胎血清和抗小鼠胎盘血清具有相似的作用。高稀释度时，抗大鼠胚胎血清更有效。分离的内细胞团培养长达72小时，但未附着于培养皿也未铺展。然而，细胞存活、增殖并分化为一层看似内胚层的细胞。
