海胆母体RNA中存在的寡聚（U）序列在受精后减少。

Oligo(U) sequences present in sea urchin maternal RNA decrease following fertilization.

作者信息

Duncan R, Humphreys T

出版信息

Dev Biol. 1983 Mar;96(1):258-62. doi: 10.1016/0012-1606(83)90326-3.

Abstract

Oligo(U) tracts were identified and measured in RNA from sea urchin eggs and embryos using a quantitative assay based on the amount of [3H]poly(A) protected from RNase T2 in duplexes with the oligo(U). The oligo(U) amounted to 0.0035% of egg RNA (0.063 X 10(-12) g/egg) and decreased to 0.0015% (0.027 X 10(-12) g/embryo) by 2 hr after fertilization. The oligo(U) tracts had a maximum size of 15-30 nucleotides and were associated with two size classes of RNA. In eggs about half were in 100 to 200 nucleotide RNA and half in mRNA-sized molecules. After fertilization, the oligo(U) in the population of large-mRNA-sized molecules was greatly reduced.

摘要

使用一种基于与寡聚（U）形成双链体时免受核糖核酸酶T2作用的[3H]多聚（A）量的定量测定法，对海胆卵和胚胎的RNA中的寡聚（U）片段进行了鉴定和测量。寡聚（U）占卵RNA的0.0035%（0.063×10⁻¹²克/卵），受精后2小时降至0.0015%（0.027×10⁻¹²克/胚胎）。寡聚（U）片段的最大长度为15至30个核苷酸，并与两种大小类别的RNA相关。在卵中，约一半存在于100至200个核苷酸的RNA中，另一半存在于mRNA大小的分子中。受精后，大mRNA大小分子群体中的寡聚（U）大大减少。