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大鼠胰岛素样生长因子II的无细胞合成

Cell-free synthesis of rat insulin-like growth factor II.

作者信息

Acquaviva A M, Bruni C B, Nissley S P, Rechler M M

出版信息

Diabetes. 1982 Jul;31(7):656-8. doi: 10.2337/diab.31.7.656.

DOI:10.2337/diab.31.7.656
PMID:6186554
Abstract

Total RNA extracted from a rat liver cell line (BRL 3A) that synthesizes rat insulin-like growth factor II (IGF-II) was translated in a reticulocyte lysate cell-free system. Incubation of the translation products with antisera to mature rat IGF-II (Mr 8700) selectively immunoprecipitated a Mr 21,600 protein. We propose that this protein represents pre-pro-IGF-II.

摘要

从合成大鼠胰岛素样生长因子II（IGF-II）的大鼠肝细胞系（BRL 3A）中提取的总RNA在网织红细胞裂解物无细胞系统中进行翻译。将翻译产物与抗成熟大鼠IGF-II（分子量8700）的抗血清一起孵育，可选择性地免疫沉淀出一种分子量为21,600的蛋白质。我们认为这种蛋白质代表前胰岛素原-IGF-II。

相似文献

1
Cell-free synthesis of rat insulin-like growth factor II.大鼠胰岛素样生长因子II的无细胞合成
Diabetes. 1982 Jul;31(7):656-8. doi: 10.2337/diab.31.7.656.
2
Biosynthesis of rat insulin-like growth factor II. I. Immunochemical demonstration of a approximately 20-kilodalton biosynthetic precursor of rat insulin-like growth factor II in metabolically labeled BRL-3A rat liver cells.大鼠胰岛素样生长因子II的生物合成。I. 代谢标记的BRL-3A大鼠肝细胞中大鼠胰岛素样生长因子II约20千道尔顿生物合成前体的免疫化学证明。
J Biol Chem. 1985 Feb 25;260(4):2570-7.
3
Synthesis of insulin-like growth factor II (IGF-II) in fetal rat tissues: translation of IGF-II ribonucleic acid and processing of pre-pro-IGF-II.胰岛素样生长因子II（IGF-II）在胎鼠组织中的合成：IGF-II核糖核酸的翻译及前胰岛素原-IGF-II的加工
Endocrinology. 1988 Feb;122(2):709-16. doi: 10.1210/endo-122-2-709.
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Biosynthesis of the low molecular weight carrier protein for insulin-like growth factors in rat liver and fibroblasts.大鼠肝脏和成纤维细胞中胰岛素样生长因子低分子量载体蛋白的生物合成。
Endocrinology. 1987 Sep;121(3):1041-50. doi: 10.1210/endo-121-3-1041.
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Biosynthesis of rat insulin-like growth factor II. II. Localization of mature rat insulin-like growth factor II (7484 daltons) to the amino terminus of the approximately 20-kilodalton biosynthetic precursor by radiosequence analysis.大鼠胰岛素样生长因子II的生物合成。II. 通过放射性序列分析将成熟的大鼠胰岛素样生长因子II（7484道尔顿）定位到约20千道尔顿生物合成前体的氨基末端。
J Biol Chem. 1985 Feb 25;260(4):2578-82.
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Insulin-like growth factor carrier proteins in neonatal and adult rat serum are immunologically different: demonstration using a new radioimmunoassay for the carrier protein from BRL-3A rat liver cells.新生大鼠和成年大鼠血清中的胰岛素样生长因子载体蛋白在免疫方面存在差异：采用针对BRL-3A大鼠肝细胞载体蛋白的新型放射免疫分析法进行的证明。
Endocrinology. 1986 May;118(5):1743-58. doi: 10.1210/endo-118-5-1743.
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Characterization of insulin-like growth factor II peptides secreted by explants of neonatal brain and of adult pituitary from rats.大鼠新生脑和成年垂体外植体分泌的胰岛素样生长因子II肽的特性研究
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Synthesis and secretion of insulin-like growth factor and its binding protein by the perfused rat liver: dependence on growth hormone status.灌注大鼠肝脏中胰岛素样生长因子及其结合蛋白的合成与分泌：对生长激素状态的依赖性。
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Neonatal rat islet cell cultures synthesize insulin-like growth factor I.新生大鼠胰岛细胞培养物可合成胰岛素样生长因子I。
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10
Coordinate developmental regulation of high and low molecular weight mRNAs for rat insulin-like growth factor II.大鼠胰岛素样生长因子II高分子量和低分子量mRNA的协调发育调控
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4519-23. doi: 10.1073/pnas.83.12.4519.

引用本文的文献

1
Insulin and insulin-like growth factor II permit nerve growth factor binding and the neurite formation response in cultured human neuroblastoma cells.胰岛素和胰岛素样生长因子II可使培养的人神经母细胞瘤细胞中的神经生长因子结合及神经突形成反应得以实现。
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2562-6. doi: 10.1073/pnas.81.8.2562.
2
Insulin, insulin-like growth factor II, and nerve growth factor effects on tubulin mRNA levels and neurite formation.胰岛素、胰岛素样生长因子II以及神经生长因子对微管蛋白mRNA水平和神经突形成的影响。
Proc Natl Acad Sci U S A. 1985 Oct;82(20):7126-30. doi: 10.1073/pnas.82.20.7126.
3
Amino acid sequence of a variant pro-form of insulin-like growth factor II.
胰岛素样生长因子II变异前体形式的氨基酸序列。
Proc Natl Acad Sci U S A. 1985 May;82(10):3169-72. doi: 10.1073/pnas.82.10.3169.
4
Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA.与大鼠胰岛素样生长因子II mRNA相关的一组转录本的发育及组织特异性表达。
Nucleic Acids Res. 1985 Feb 25;13(4):1119-34. doi: 10.1093/nar/13.4.1119.
5
Insulin-like growth factor II (IGF II) in human brain: regional distribution of IGF II and of higher molecular mass forms.人脑中的胰岛素样生长因子II（IGF II）：IGF II及其高分子量形式的区域分布
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2153-7. doi: 10.1073/pnas.82.7.2153.
6
Synthesis of somatomedin C/insulin-like growth factor I by human placenta.人胎盘合成生长调节素C/胰岛素样生长因子I。
Mol Biol Rep. 1986;11(4):231-6. doi: 10.1007/BF00419602.
7
Coordinate developmental regulation of high and low molecular weight mRNAs for rat insulin-like growth factor II.大鼠胰岛素样生长因子II高分子量和低分子量mRNA的协调发育调控
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4519-23. doi: 10.1073/pnas.83.12.4519.
8
A new member of the ras gene superfamily identified in a rat liver cell line.在大鼠肝细胞系中鉴定出的ras基因超家族的一个新成员。
Nucleic Acids Res. 1988 Nov 11;16(21):9979-93. doi: 10.1093/nar/16.21.9979.
9
Insulin-like growth factor II in human adrenal pheochromocytomas and Wilms tumors: expression at the mRNA and protein level.胰岛素样生长因子II在人肾上腺嗜铬细胞瘤和肾母细胞瘤中的表达：mRNA和蛋白质水平的表达情况
Proc Natl Acad Sci U S A. 1987 Feb;84(4):1104-6. doi: 10.1073/pnas.84.4.1104.
10
Somatomedins: chemical and functional characteristics of the different molecular forms.生长调节素：不同分子形式的化学和功能特性
J Endocrinol Invest. 1989 Apr;12(4):279-93. doi: 10.1007/BF03349988.