Ferri G L, Adrian T E, Ghatei M A, O'Shaughnessy D J, Probert L, Lee Y C, Buchan A M, Polak J M, Bloom S R
Gastroenterology. 1983 Apr;84(4):777-86.
The localization and distribution of regulatory peptides was studied in separated epithelium, lamina propria, submucosa, and external muscular layer from 16 specimens of human bowel. Immunoreactive enteroglucagon, gastric inhibitory polypeptide, and neurotensin were almost confined to the epithelial fraction (97.5 +/- 2.2%, 97.5 +/- 4.2%, and 99.3 +/- 1.1% of their respective total content, mean +/- SEM) and were only localized in endocrine cells. Vasoactive intestinal polypeptide-, substance P-, and bombesinlike peptides were virtually restricted to the nonepithelial layers (99.6 +/- 0.2%, 99.6 +/- 0.2%, and 100%) and were demonstrated exclusively in nerves. A particularly rich vasoactive intestinal polypeptide- and substance P-immunoreactive nerve supply was seen in the nonepithelial mucosa, which contained the highest concentrations of these peptides, while bombesin was mainly recovered from the external muscle (87.7 +/- 2.7%). Somatostatin, measured with an antiserum highly specific for somatostatin-14, was found throughout the wall, mainly in the epithelium (39.9 +/- 5.2%) and lamina propria (29.5 +/- 5.9%), but could be immunostained only in endocrine cells.
在取自16例人体肠段标本的分离上皮、固有层、黏膜下层和外肌层中，研究了调节肽的定位和分布。免疫反应性肠高血糖素、胃抑制多肽和神经降压素几乎局限于上皮部分（分别占各自总含量的97.5±2.2%、97.5±4.2%和99.3±1.1%，均值±标准误），且仅定位于内分泌细胞。血管活性肠肽、P物质和蛙皮素样肽实际上局限于非上皮层（分别为99.6±0.2%、99.6±0.2%和100%），且仅在神经中显示。在非上皮黏膜中可见特别丰富的血管活性肠肽和P物质免疫反应性神经支配，该层含有这些肽的最高浓度，而蛙皮素主要从外肌层回收（87.7±2.7%）。用对生长抑素-14高度特异的抗血清检测的生长抑素在肠壁各处均有发现，主要在上皮（39.9±5.2%）和固有层（29.5±5.9%），但仅在内分泌细胞中可进行免疫染色。
