Kameyama T, Nabeshima T, Itoh J
Nihon Yakurigaku Zasshi. 1982 Dec;80(6):525-35.
To study the auditory impairment induced by prenatal administration of aminoglycosides in the offspring, the shuttle box method to measure the auditory threshold of rats (Kameyama et al., Folia pharmacol. japon. 77, 15, 1981) was employed. Four groups of pregnant rats were administered 200 mg/kg kanamycin sulfate (KM), 200 mg/kg dihydrostreptomycin sulfate (DHSM), 100 mg/kg neomycin sulfate (NM), or 1 ml/kg saline intramuscularly from the 10th to the 19th day of pregnancy. The auditory threshold of the offspring could be measured by the shuttle box method in about 90% of the live born rats at the age of 100 days. The auditory thresholds of the groups were as follows (mean +/- S.E.): saline group, 53.8 +/- 0.6 dB (N = 36); KM group, 63.8 +/- 1.1 dB (N = 34); DHSM group, 60.0 +/- 1.2 dB (N = 29); NM group, 62.4 +/- 1.2 dB (N = 24). Auditory thresholds of drug-treated groups were significantly higher than that of the saline group. However, no increase in the auditory threshold of the mother rat was detected after treatment with aminoglycosides. In addition, the experimental procedure of the shuttle box method is very easy, and the auditory threshold of a large number of rats could be measured in a short period. These findings suggest that this method is a very useful one for screening for auditory impairment induced by prenatal drug treatment in rat offspring.
为研究孕期给予氨基糖苷类药物对后代听觉损伤的影响，采用穿梭箱法测量大鼠听觉阈值（龟山等人，日本药理学杂志77, 15, 1981）。将四组怀孕大鼠于妊娠第10天至第19天分别肌肉注射200mg/kg硫酸卡那霉素（KM）、200mg/kg硫酸双氢链霉素（DHSM）、100mg/kg硫酸新霉素（NM）或1ml/kg生理盐水。在100日龄时，约90%的存活仔鼠可用穿梭箱法测量听觉阈值。各实验组的听觉阈值如下（平均值±标准误）：生理盐水组，53.8±0.6dB（N = 36）；KM组，63.8±1.1dB（N = 34）；DHSM组，60.0±1.2dB（N = 29）；NM组，62.4±1.2dB（N = 24）。药物处理组的听觉阈值显著高于生理盐水组。然而，氨基糖苷类药物处理后未检测到母鼠听觉阈值升高。此外，穿梭箱法的实验操作非常简便，可在短时间内测量大量大鼠的听觉阈值。这些结果表明，该方法对于筛查孕期药物处理对大鼠后代听觉损伤非常有用。
