Jackson D, Walker-Smith J A, Phillips A D
Histopathology. 1982 Nov;6(6):689-702. doi: 10.1111/j.1365-2559.1982.tb02764.x.
The passive permeability of the small intestinal mucosa in childhood was investigated by studying the penetration of two light- and electron-dense tracer molecules (ruthenium red mol. wt = 1000 and horseradish peroxidase mol. wt = 40 000) into fixed biopsy specimens. Ruthenium red confirmed the presence of damaged and extruding epithelial cells in normal mucosa and showed a significant increase in such cells in abnormal mucosa. Horseradish peroxidase demonstrated that antigenically-sized molecules could enter a proportion of these cells and significantly more cells were penetrated in abnormal mucosa, reflecting the ruthenium red results. Thus, anatomical pathways for passive diffusion of antigen through damaged or extruding cells exist in normal and, to a greater extent, in abnormal small intestinal mucosae in childhood. Further study using living tissue may reveal the importance of this phenomenon in relation to the active uptake of antigen.
通过研究两种具有光密度和电子密度的示踪分子（钌红，分子量 = 1000；辣根过氧化物酶，分子量 = 40000）在固定活检标本中的渗透情况，对儿童小肠黏膜的被动通透性进行了研究。钌红证实正常黏膜中存在受损和脱落的上皮细胞，并且显示异常黏膜中此类细胞显著增多。辣根过氧化物酶表明，抗原大小的分子能够进入一部分此类细胞，且异常黏膜中有更多细胞被穿透，这与钌红的结果相符。因此，在正常以及在更大程度上在儿童异常小肠黏膜中，存在抗原通过受损或脱落细胞进行被动扩散的解剖学途径。使用活组织进行的进一步研究可能会揭示这一现象在抗原主动摄取方面的重要性。
