Belardelli F, Gresser I, Maury C, Maunoury M T
Int J Cancer. 1982 Dec 15;30(6):813-20. doi: 10.1002/ijc.2910300621.
Interferon-sensitive (745) and interferon-resistant (3C1-8) Friend leukemia cells (FLC) are highly tumorigenic for DBA/2 mice. The phenotype of interferon sensitivity or resistance does not change with in vivo passage. Daily administration of mouse interferon markedly enhanced the survival time of mice injected with either 745 or 3C1-8 cells. Use of quantitative methods for determining the number of FLC (colony formation in agarose and immunofluorescence) permitted us to show that potent, partially purified or highly purified mouse interferon (s.a. 0.5 to 1 X 10(9) u/mg protein) induced a 100- to 1,000-fold decrease in the number of tumor cells in the peritoneal cavity in the days following inoculation of 745 or 3C1-8 cells. Interferon decreased the number of FLC even when treatment was initiated at a time when tumor cells were multiplying exponentially in the peritoneal cavity. There was no evidence that interferon acted as an inducer of FLC differentiation in vivo. The finding that interferon was equally effective in mice inoculated with interferon-resistant cells as in mice inoculated with interferon-sensitive cells suggests that in this experimental system interferon does not act directly on the tumor cells, but that the interferon-induced antitumor activity is mediated by the host.
干扰素敏感型（745）和干扰素耐药型（3C1 - 8）的弗氏白血病细胞（FLC）对DBA/2小鼠具有高度致瘤性。干扰素敏感性或耐药性的表型不会随着体内传代而改变。每日注射小鼠干扰素可显著延长注射745或3C1 - 8细胞的小鼠的存活时间。使用定量方法测定FLC的数量（琼脂糖中的集落形成和免疫荧光）使我们能够表明，高效、部分纯化或高度纯化的小鼠干扰素（比活性为0.5至1×10⁹单位/毫克蛋白质）在接种745或3C1 - 8细胞后的几天内，可使腹腔内肿瘤细胞数量减少100至1000倍。即使在肿瘤细胞在腹腔内呈指数增殖时开始治疗，干扰素也能减少FLC的数量。没有证据表明干扰素在体内作为FLC分化的诱导剂起作用。干扰素对接种干扰素耐药细胞的小鼠和接种干扰素敏感细胞的小鼠同样有效的这一发现表明，在这个实验系统中，干扰素并不直接作用于肿瘤细胞，而是干扰素诱导的抗肿瘤活性是由宿主介导的。
