Corstvet R E, Gentry M J, Newman P R, Rummage J A, Confer A W
J Clin Microbiol. 1982 Dec;16(6):1123-6. doi: 10.1128/jcm.16.6.1123-1126.1982.
Extracellular capsular material was demonstrated on early log-phase cells of Pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. The presence of this material was shown to be age dependent. Wide capsules were demonstrable on cells from 2- to 12-h cultures, whereas cells from 16- to 22-h cultures had very little cell-associated capsular material. The Maneval technique most clearly demonstrated the presence of capsules on cells from young (6-h) cultures when compared with other capsule staining techniques.
通过荧光抗体和几种荚膜染色技术，在溶血巴斯德氏菌1型早期对数期细胞上证实了细胞外荚膜物质。这种物质的存在显示出与菌龄有关。在培养2至12小时的细胞上可观察到宽大的荚膜，而培养16至22小时的细胞与细胞相关的荚膜物质很少。与其他荚膜染色技术相比，马尼瓦尔技术最清楚地显示了年轻（6小时）培养物细胞上荚膜的存在。
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