溶血巴氏杆菌白细胞毒素的溶血活性。
Hemolytic activity of the Pasteurella haemolytica leukotoxin.
作者信息
Murphy G L, Whitworth L C, Clinkenbeard K D, Clinkenbeard P A
机构信息
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
出版信息
Infect Immun. 1995 Aug;63(8):3209-12. doi: 10.1128/iai.63.8.3209-3212.1995.
Abstract
A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demonstrated the typical beta-hemolytic phenotype on sheep and rabbit blood agar, whereas the mutant did not.
摘要
通过等位基因置换构建了一株无法产生白细胞毒素的溶血巴斯德菌突变体。野生型溶血巴斯德菌的浓缩培养上清液中含有102 kDa的白细胞毒素蛋白，可裂解牛淋巴瘤细胞和绵羊红细胞，而突变体的培养上清液则无此作用。野生型溶血巴斯德菌在绵羊和兔血琼脂上表现出典型的β-溶血表型，而突变体则没有。
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