Roseleur O J, van Gent C M
Clin Chim Acta. 1978 Jan 2;82(1-2):13-23. doi: 10.1016/0009-8981(78)90021-9.
A simplified method for the quantitative isolation and determination of sterols and bile acids for application in sterol balance studies in humans consuming mixed general food is described. The recovery of steroids from diets and from faeces was found to be quantitative. By application of temperature programmed gas-liquid chromatography (GLC), thin-layer chromatographic (TLC) pre-separation could be omitted from the multistep analytical procedure. Furthermore, it was possible to use simple internal standards, 5alpha-cholestane and/or 5beta-cholanic acid, instead of labeled compounds for the calculation of overall recoveries.
本文描述了一种简化方法，用于定量分离和测定甾醇和胆汁酸，该方法适用于食用混合普通食物的人类甾醇平衡研究。发现从饮食和粪便中回收类固醇的量是定量的。通过应用程序升温气相色谱法（GLC），多步分析程序中可以省略薄层色谱（TLC）预分离步骤。此外，计算总回收率时，可以使用简单的内标物5α-胆甾烷和/或5β-胆酸，而不是标记化合物。
