Happonen R P, Viander M
J Oral Pathol. 1982 Dec;11(6):417-25. doi: 10.1111/j.1600-0714.1982.tb00185.x.
Eight formalin-fixed and paraffin-embedded biopsies with a histopathology suggestive of actinomycosis, were tested with fluorescent antibody technique using antiserum specific against Actinomyces israelii. The method was found effective even in decalcified tissue material. Positive fluorescent staining resulted, not only in the colonies but also in the surrounding cells, suggesting the presence of intracellular Actinomyces antigens. The possibility of retrospective biopsy study is very helpful in cases of prolonged infections. Grocott's stain was found to be most effective conventional method for screening suspected actinomycosis.
对八份经福尔马林固定、石蜡包埋且组织病理学提示放线菌病的活检样本，采用针对以色列放线菌的抗血清，通过荧光抗体技术进行检测。结果发现该方法即便在脱钙组织材料中也有效。阳性荧光染色不仅出现在菌落中，也出现在周围细胞中，提示存在细胞内放线菌抗原。回顾性活检研究的可能性在长期感染病例中非常有帮助。格罗科特染色被发现是筛查疑似放线菌病最有效的传统方法。
