Stunnenberg H G, van den Broek H W
Microbios. 1982;35(141-142):197-207.
RNA synthesis was studied in protoplasts from Aspergillus nidulans, which remained metabolically active for several days under the appropriate conditions. It is concluded, that at certain actinomycin D concentrations the ribosomal RNA synthesis can be selectively inhibited. However, alpha-amanitin at concentrations up to 400 micrograms/ml neither influenced the total nor the RNA polymerase II directed RNA synthesis. It was possible to discriminate between the RNA polymerase I or II directed RNA synthesis due to the presence of poly(A) tails attached to at least part of the RNA, presumably messenger RNAs, or their precursors.
对来自构巢曲霉的原生质体中的RNA合成进行了研究，在适当条件下，这些原生质体在数天内保持代谢活性。得出的结论是，在某些放线菌素D浓度下，核糖体RNA合成可被选择性抑制。然而，浓度高达400微克/毫升的α-鹅膏蕈碱既不影响总RNA合成，也不影响RNA聚合酶II指导的RNA合成。由于至少部分RNA（可能是信使RNA或其前体）上附着有聚（A）尾，因此能够区分RNA聚合酶I或II指导的RNA合成。
