环磷酸腺苷可稳定一类受发育调控的盘基网柄菌信使核糖核酸。

Cyclic AMP stabilizes a class of developmentally regulated Dictyostelium discoideum mRNAs.

作者信息

Mangiarotti G, Ceccarelli A, Lodish H F

出版信息

Nature. 1983;301(5901):616-8. doi: 10.1038/301616a0.

DOI:10.1038/301616a0

Abstract

The stability of mRNA is an important facet of the regulation of protein synthesis. In mammalian cells most mRNAs have long half-lives (5-15 hours) but a substantial fraction are much less stable. There are few examples where the stability of a particular mRNA or class of mRNAs is specifically affected by environmental or developmental stimuli. Certain hormones cause specific stabilization of mRNAs species and preferential mRNA stability is important in the accumulation of globin and myosin mRNAs during the terminal stages of erythropoesis or myogenesis, respectively. Disaggregation of Dictyostelium discoideum aggregates induces the specific destabilization of a large class of developmentally regulated mRNAs; thus, this system is an excellent one in which to determine how such controls are effected. Here we show that addition of cyclic AMP to disaggregated cells specifically prevents the destabilization of these mRNAs.

摘要

信使核糖核酸（mRNA）的稳定性是蛋白质合成调控的一个重要方面。在哺乳动物细胞中，大多数mRNA具有较长的半衰期（5 - 15小时），但相当一部分mRNA的稳定性要低得多。很少有特定mRNA或一类mRNA的稳定性受到环境或发育刺激特异性影响的例子。某些激素会导致特定mRNA种类的稳定，并且优先的mRNA稳定性分别在红细胞生成或肌生成的终末阶段血红蛋白和肌球蛋白mRNA的积累中很重要。盘基网柄菌聚集体的解体诱导了一大类发育调控mRNA的特异性去稳定化；因此，这个系统是确定此类调控如何实现的绝佳系统。在这里，我们表明向解体的细胞中添加环磷酸腺苷（cAMP）可特异性地防止这些mRNA的去稳定化。