Nagy J I, Goedert M, Hunt S P, Bond A
Neuroscience. 1982;7(12):3137-51. doi: 10.1016/0306-4522(82)90236-6.
The nature of the association of substance P (SP) with taste buds in the rat tongue was investigated by immunohistochemical and radioimmunoassay techniques. Both the circumvallate and fungiform papillae were found to receive a rich innervation by substance P-containing fibres. Although these fibres were closely associated with the taste buds in these structures, they assumed a perigemmal rather than an intragemmal location. Bilateral lesions of the glossopharyngeal nerve resulted in the depletion of taste buds from the vallate papilla and a large reduction in substance P immunoreactive fibres in this area. Lesions of the chorda tympani, which led to the degeneration of taste buds in fungiform papillae, had no effect on the immunohistochemical appearance of substance P in these papilla or on the substance P levels in the anterior part of the tongue. Lesions of the mandibular division of the trigeminal nerve or neonatal capsaicin treatment had no effect on the structural integrity of taste buds in fungiform papillae but led to the depletion of substance P-immunoreactive fibres from these papillae. Both of these procedures caused a 71% reduction in the substance P content of the anterior tongue, ipsilaterally after the nerve lesion and bilaterally after capsaicin treatment. The results are discussed in relation to the possible functional role of substance P-containing fibres within nerves supplying taste structures of the tongue.
采用免疫组织化学和放射免疫分析技术，研究了大鼠舌中P物质（SP）与味蕾的关联性质。发现轮廓乳头和菌状乳头均接受含P物质纤维的丰富神经支配。尽管这些纤维与这些结构中的味蕾紧密相关，但它们位于味蕾周围而非味蕾内部。双侧舌咽神经损伤导致轮廓乳头的味蕾缺失，且该区域中P物质免疫反应性纤维大量减少。鼓索神经损伤导致菌状乳头中的味蕾退化，但对这些乳头中P物质的免疫组织化学表现或舌前部的P物质水平没有影响。三叉神经下颌支损伤或新生大鼠辣椒素处理对菌状乳头中味蕾的结构完整性没有影响，但导致这些乳头中P物质免疫反应性纤维缺失。这两种处理均使舌前部的P物质含量降低71%，神经损伤后为同侧降低，辣椒素处理后为双侧降低。结合含P物质纤维在供应舌味觉结构的神经中的可能功能作用，对结果进行了讨论。
