Pfeffer P F, Thorsby E, Hirschberg H
Transplantation. 1983 Feb;35(2):156-60. doi: 10.1097/00007890-198302000-00009.
Patients with well functioning kidney grafts from one HLA haplotype-mismatched related donors were studied 2 to 10 years after transplantation. No direct cell-mediated lympholysis (CML) toward donor cells was found. After in vitro sensitization in mixed lymphocyte culture, the recipient effector cells were not able to significantly kill donor target cells at an effector to target cell ratio of 50:1, while the HLA-A,B,D/DR-identical sibling of the recipient could generate strong cytotoxicity toward the donor at similar effector to target cell ratios. Nevertheless, the patient developed strong cytotoxicity toward third-party targets. Our findings indicate in vivo depletion of cytotoxic cells with specificity for donor antigens. No correlation between the mixed lymphocyte culture (MLC) response and the ability to generate cytotoxic cells could be found in recipient-donor combinations. In looking for in vivo generated suppressor cells toward the donor, a moderate suppression was found in three of six patients studied.
对来自一位 HLA 单倍型不匹配的相关供体且移植肾功能良好的患者进行了移植后 2 至 10 年的研究。未发现针对供体细胞的直接细胞介导淋巴细胞溶解（CML）。在混合淋巴细胞培养中进行体外致敏后，受体效应细胞在效应细胞与靶细胞比例为 50:1 时无法显著杀伤供体靶细胞，而受体 HLA - A、B、D/DR 相同的同胞在相似的效应细胞与靶细胞比例下可对供体产生强烈的细胞毒性。然而，患者对第三方靶标产生了强烈的细胞毒性。我们的研究结果表明体内具有针对供体抗原特异性的细胞毒性细胞被耗竭。在受体 - 供体组合中未发现混合淋巴细胞培养（MLC）反应与产生细胞毒性细胞的能力之间存在相关性。在寻找体内针对供体产生的抑制细胞时，在所研究的 6 名患者中有 3 名发现了适度的抑制作用。
