Siefring G E, Riabov D K, Wehrly J A
Clin Chem. 1983 Apr;29(4):614-7.
A method for determination of functional fibrinogen has been developed for the Du Pont aca discrete clinical analyzer. This fully automated assay is based on the rate of fibrin turbidity formation when thrombin is added to the test sample. The rate of this reaction is enhanced by added calcium chloride and dextran. The assay range is from 0.50 to 8.00 g/L. Within-run reproducibility studies at the medical decision level of 1.0 g/L showed a CV of 2.5%. There was no interference by heparin up to 10 USP units per milliliter of plasma.
已为杜邦aca离散临床分析仪开发了一种测定功能性纤维蛋白原的方法。这种全自动检测方法基于向测试样品中加入凝血酶时纤维蛋白浊度形成的速率。添加氯化钙和葡聚糖可提高该反应的速率。检测范围为0.50至8.00 g/L。在医学判定水平1.0 g/L下进行的批内重复性研究显示变异系数为2.5%。每毫升血浆中肝素含量高达10 USP单位时无干扰。
