Trowbridge I S, Mazauskas C
Eur J Immunol. 1976 Aug;6(8):557-62. doi: 10.1002/eji.1830060806.
The immunological properties of two murine thymus-dependent (T) lymphocyte surface glycoproteins, T200 and T25, were investigated. T200 is a lymphocyte-specific antigen with a high degree of species specificity. It shares antigenic determinants with molecules present on thymus-independent (B) lymphocytes. T25 has antigenic determinants which cross-react with antigens on mouse brain, rat thymocytes and rat brain. An antiserum against a purified rat brain glycoprotein which carries Thy-1.1 reacts with T25. Absorption of this antiserum with BALB/c thymocytes or brain homogenate produces a Thy-1.1 specific serum which reacts with T25 from AKR/J thymocytes but not with T25 from AKR/Cum thymocytes. These results confirm that T25 is the molecule on the surface of mouse T cells which carries the Thy-1 antigen. T25 also carries antigenic determinants, recognized by anti-thymocyte serum (ATS), which were found on secondary mouse embryo fibroblasts and untransformed fibroblast cell lines but which were not detected on fibroblast cell lines transformed with murine sarcoma virus (MSV) or with Simian virus 40 (SV40).
对两种小鼠胸腺依赖性（T）淋巴细胞表面糖蛋白T200和T25的免疫特性进行了研究。T200是一种具有高度物种特异性的淋巴细胞特异性抗原。它与非胸腺依赖性（B）淋巴细胞上存在的分子共享抗原决定簇。T25具有与小鼠脑、大鼠胸腺细胞和大鼠脑上的抗原发生交叉反应的抗原决定簇。一种针对携带Thy-1.1的纯化大鼠脑糖蛋白的抗血清与T25发生反应。用BALB/c胸腺细胞或脑匀浆吸收该抗血清可产生一种Thy-1.1特异性血清，该血清与AKR/J胸腺细胞的T25发生反应，但不与AKR/Cum胸腺细胞的T25发生反应。这些结果证实T25是小鼠T细胞表面携带Thy-1抗原的分子。T25还携带被抗胸腺细胞血清（ATS）识别的抗原决定簇，这些抗原决定簇在第二代小鼠胚胎成纤维细胞和未转化的成纤维细胞系上被发现，但在用鼠肉瘤病毒（MSV）或猿猴病毒40（SV40）转化的成纤维细胞系上未检测到。
