Baccaglini P I, Hogan P G
Proc Natl Acad Sci U S A. 1983 Jan;80(2):594-8. doi: 10.1073/pnas.80.2.594.
Sensory neurons were dissociated from trigeminal ganglia or from dorsal root ganglia of rats, grown in culture, and examined for expression of properties of pain sensory cells. Many sensory neurons in culture are excited by low concentrations of capsaicin, reportedly a selective stimulus for pain sensory neurons. Many are excited by bradykinin, sensitized by prostaglandin E2, or specifically stained by an antiserum against substance P. These experiments provide a basis for the study of pain mechanisms in cell culture.
感觉神经元从大鼠的三叉神经节或背根神经节中分离出来，在培养物中生长，并检测其疼痛感觉细胞特性的表达。培养中的许多感觉神经元会被低浓度的辣椒素所兴奋，据报道辣椒素是疼痛感觉神经元的一种选择性刺激物。许多神经元会被缓激肽兴奋，被前列腺素E2致敏，或被抗P物质抗血清特异性染色。这些实验为在细胞培养中研究疼痛机制提供了基础。
