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通过流式细胞术进行细菌鉴定

Bacterial characterization by flow cytometry.

作者信息

Van Dilla M A, Langlois R G, Pinkel D, Yajko D, Hadley W K

出版信息

Science. 1983 May 6;220(4597):620-2. doi: 10.1126/science.6188215.

DOI:10.1126/science.6188215
PMID:6188215
Abstract

Bacteria were analyzed in a dual-beam flow cytometer after double staining with the fluorescent dyes chromomycin A3 and Hoechst 33258, which bind preferentially to DNA that is rich in guanine-cytosine and adenine-thymine, respectively. The measurements were indicative of the cellular DNA content and base composition, cell concentration, and proliferative state of the population. The ratio of the chromomycin A3 signal to the Hoechst 33258 signal increased with the guanine-cytosine content of the cellular DNA for the six cultured species measured, following expectation. Bacteria in urine from patients with urinary tract infections were characterized without interference from host cell DNA, debris, or other particulates.

摘要

在用荧光染料放线菌素A3和Hoechst 33258进行双重染色后，使用双光束流式细胞仪对细菌进行分析，这两种染料分别优先结合富含鸟嘌呤-胞嘧啶和腺嘌呤-胸腺嘧啶的DNA。这些测量结果可反映细胞的DNA含量、碱基组成、细胞浓度以及群体的增殖状态。在所测量的六个培养物种中，放线菌素A3信号与Hoechst 33258信号的比值随着细胞DNA中鸟嘌呤-胞嘧啶含量的增加而增加，符合预期。尿路感染患者尿液中的细菌得到了表征，且不受宿主细胞DNA、碎片或其他颗粒的干扰。

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1
Bacterial characterization by flow cytometry.通过流式细胞术进行细菌鉴定
Science. 1983 May 6;220(4597):620-2. doi: 10.1126/science.6188215.
2
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Fluorescence spectra of DNA dyes measured in a flow cytometer.在流式细胞仪中测量的DNA染料的荧光光谱。
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