Fishman J H
Biochem Biophys Res Commun. 1983 Feb 10;110(3):713-8. doi: 10.1016/0006-291x(83)91019-7.
Treating mature rat uterine cytosol with dextran coated charcoal (DCC) for 2 hrs at 0-4C in the absence of ligand causes the subsequently formed receptor-estradiol complex to be stable at 37C. Receptor binding is increased by the DCC treatment for uteri excised at metestrus or diestrus but remains nearly unchanged for uteri obtained at proestrus or estrus. Results suggest that the DCC removes or inactivates factor(s) present in the cytosol which render the receptor complex thermolabile.
在无配体存在的情况下，于0 - 4℃用葡聚糖包被活性炭（DCC）处理成熟大鼠子宫胞质溶胶2小时，会使随后形成的受体 - 雌二醇复合物在37℃时保持稳定。对于在动情后期或间情期切除的子宫，DCC处理会增加受体结合，但对于在动情前期或动情期获得的子宫，受体结合几乎保持不变。结果表明，DCC去除或使胞质溶胶中存在的使受体复合物热不稳定的因子失活。
